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Image Search Results
Journal: PLoS ONE
Article Title: Identification of GAD65 AA 114-122 reactive 'memory-like' NK cells in newly diagnosed Type 1 diabetic patients by HLA-class I pentamers
doi: 10.1371/journal.pone.0189615
Figure Lengend Snippet: PBMC from healthy blood donors and T1D patients, were GAD65 AA 114–122 peptide stimulated, stained with CD3, CD8 and GAD65 AA 114–122 HLA A*02:01 pentamers, then analyzed by FACS to determine the percentages of GAD65 pentamer reactive cells in CD3 + CD8 bright , CD3 + CD8 dull , total CD3 + CD8 + and CD3 - CD8 dull subsets (see ). In the graph, horizontal bars represent the mean percentage of CD3 - CD8 dull GAD65 AA 114–122 reactive cells and each symbol represents an individual: circle dots represents the normal and square dots the diabetic. Percentages of CD3 - CD8 dull GAD65 reactive cells refer to analyzed events within flow-cytometry gates as shown in representative dot plots in . GAD65 + = reactive with PE-labelled GAD65 AA 114–122 pentamer. N = number of T1D patients and HD controls analyzed.
Article Snippet: A subsequent staining procedure for phenotypic characterization of CD3 - CD8 dull GAD65 AA 114–122 pentamer reactive T cells was implemented by adding the following antibodies: mAb anti-human CD8 FITC (1:20, BD),
Techniques: Staining, Flow Cytometry
Journal: PLoS ONE
Article Title: Identification of GAD65 AA 114-122 reactive 'memory-like' NK cells in newly diagnosed Type 1 diabetic patients by HLA-class I pentamers
doi: 10.1371/journal.pone.0189615
Figure Lengend Snippet: Relative percentages of CD3 + CD8 bright (A), CD3 + CD8 dull (B), total CD3 + CD8 + (C) and CD3 - CD8 dull cells (D) in PBMC of newly diagnosed (ND, circle dots) vs long-term T1D patients (LT, square dots); horizontal bars, average values are reported.
Article Snippet: A subsequent staining procedure for phenotypic characterization of CD3 - CD8 dull GAD65 AA 114–122 pentamer reactive T cells was implemented by adding the following antibodies: mAb anti-human CD8 FITC (1:20, BD),
Techniques:
Journal: PLoS ONE
Article Title: Identification of GAD65 AA 114-122 reactive 'memory-like' NK cells in newly diagnosed Type 1 diabetic patients by HLA-class I pentamers
doi: 10.1371/journal.pone.0189615
Figure Lengend Snippet: Relative percentages of CD3 - CD8 dull CD56 + and CD3 - CD8 dull CD56 + ILT2 + GAD65 AA 114–122 pentamer reactive cells in T1D patients vs healthy controls after GAD65 peptide stimulation. (A) Representative experiment showing the flow-cytometry gate strategy; (B) Summary of results of CD3 - CD8 dull CD56 + GAD65 AA 114–122 pentamer reactive cells obtained in 15 healthy donors (circle dots) and 15 T1D patients (square dots); (C) Summary of results of CD3 - CD8 dull CD56 + ILT2 + GAD65 AA 114–122 pentamer reactive cells. Horizontal bars, average values are reported.
Article Snippet: A subsequent staining procedure for phenotypic characterization of CD3 - CD8 dull GAD65 AA 114–122 pentamer reactive T cells was implemented by adding the following antibodies: mAb anti-human CD8 FITC (1:20, BD),
Techniques: Flow Cytometry
Journal: PLoS ONE
Article Title: Identification of GAD65 AA 114-122 reactive 'memory-like' NK cells in newly diagnosed Type 1 diabetic patients by HLA-class I pentamers
doi: 10.1371/journal.pone.0189615
Figure Lengend Snippet: The percentage of CD3 - CD56 + cells (A) and of CD3 - CD56 + ILT2 + cells (B) were assessed by flow cytometric analyses of IL-2 alone treated PBMC. NK cell phenotype of 14 healthy donors (HD, circle plots) was compared with that of 14 T1D patients (square dots); horizontal bars, average values are shown. No significant differences are reported (KS test, unpaired t test).
Article Snippet: A subsequent staining procedure for phenotypic characterization of CD3 - CD8 dull GAD65 AA 114–122 pentamer reactive T cells was implemented by adding the following antibodies: mAb anti-human CD8 FITC (1:20, BD),
Techniques:
Journal: PLoS ONE
Article Title: Identification of GAD65 AA 114-122 reactive 'memory-like' NK cells in newly diagnosed Type 1 diabetic patients by HLA-class I pentamers
doi: 10.1371/journal.pone.0189615
Figure Lengend Snippet: Degranulation of CD3 - CD56 + ILT2 + NK cells of PBMC from T1D patients, expanded with GAD65 AA 114–122 or FLU peptides, measured as CD107a cell-surface expression following stimulation with APCs, either left unpulsed or GAD65 AA 114–122 peptide-pulsed. (A) A representative experiment out of two performed is shown. K562 cells were used as positive control. The percentage of CD3 - CD56 + CD107a + NK cells (upper panel) and CD3 - CD56 + CD107a + ILT2 + (lower panel) is indicated for each condition. (B) Summary of CD3 - CD56 + CD107a + and (C) CD3 - CD56 + ILT2 + CD107a + NK cell percentage of four T1D PBMC, expanded with GAD65 AA 114–122 or FLU peptides, following stimulation with APCs, either left unpulsed (circle dots) or GAD65 AA 114–122 (GAD65)-pulsed (square dots); horizontal bars, average values are reported.
Article Snippet: A subsequent staining procedure for phenotypic characterization of CD3 - CD8 dull GAD65 AA 114–122 pentamer reactive T cells was implemented by adding the following antibodies: mAb anti-human CD8 FITC (1:20, BD),
Techniques: Expressing, Positive Control
Journal: PLoS ONE
Article Title: Identification of GAD65 AA 114-122 reactive 'memory-like' NK cells in newly diagnosed Type 1 diabetic patients by HLA-class I pentamers
doi: 10.1371/journal.pone.0189615
Figure Lengend Snippet: Degranulation of CD3 - CD56 + ILT2 + NK cells of PBMC from HD patients, expanded with GAD65 AA 114–122 peptide, measured as CD107a cell-surface expression following stimulation with APCs, either left unpulsed or GAD65 AA 114–122 peptide-pulsed. A representative experiment out of two performed is shown. K562 cells were used as positive control. The percentage of CD3 - CD56 + CD107a + NK cells (upper panel) and of CD3 - CD56 + CD107a + ILT2 + (lower panel) is indicated for each condition.
Article Snippet: A subsequent staining procedure for phenotypic characterization of CD3 - CD8 dull GAD65 AA 114–122 pentamer reactive T cells was implemented by adding the following antibodies: mAb anti-human CD8 FITC (1:20, BD),
Techniques: Expressing, Positive Control